E.Z.N.A.? Plasmid Mini Kit I 從1-5ml過(guò)夜培養的菌液中純化高達35μg質(zhì)粒DNA

該試劑盒采用經(jīng)典的堿裂解法和硅膠柱純化方式，適合于從1.5-5.0ml培養過(guò)夜的大腸桿菌(DH5α, JM109)等菌液中抽提5-35μg高純度的質(zhì)粒DNA。

操作者可在30分鐘內完成多個(gè)樣品的質(zhì)粒提取工作。純化的質(zhì)?？梢灾苯佑糜谧詣?dòng)測序、限制性?xún)惹忻该盖?、PCR、標記等實(shí)驗。

從1.5-5.0ml培養過(guò)夜的菌液中收集細菌后，采用經(jīng)典的堿裂解法裂解細菌，離心去除染色體DNA和蛋白質(zhì)，上清液轉移至硅膠柱，離心吸附質(zhì)粒DNA，經(jīng)兩種溶液洗滌去除殘余蛋白質(zhì)和各種雜質(zhì)，用Elution Buffer洗脫出質(zhì)粒DNA。

產(chǎn)品特性與優(yōu)點(diǎn) 安全-無(wú)酚氯仿等危險的有機物抽提 簡(jiǎn)單-硅膠柱過(guò)濾操作 快速-在30分鐘內完成多個(gè)樣品的抽提工作 超值-性?xún)r(jià)比高

送硅膠柱、 離心管 等耗材各一包.

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The E.Z.N.A.? Plasmid Mini Kit I is designed to isolate up to 30 μg of high-quality plasmid DNA from 1-5 mL bacterial cultures in 30 minutes or less. Plasmid DNA purification is simplified with HiBind? Mini Column technology into three quick steps: bind, wash, and elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, and manual/automated fluorescent DNA sequencing. E.Z.N.A.? Plasmid Mini Kit I is available in V- Spin and Q-Spin column formats. ?V Spin Column Formats have an attached lid while Q Spin Columns do not. ?Both versions can be used for centrifugation or vacuum protocols.

• Rapid – Purification of plasmid DNA in 30 minutes or less
• Safe – No phenol/chloroform extractions
• Versatile – Spin and vacuum formats available
• High-quality – DNA is suitable for downstream application