地高辛發(fā)光檢測試劑盒DIG Luminescent Detection Kit

貨號:

11363514910

品牌:

Roche

規格:

1 kit

價(jià)格:

詢(xún)價(jià)

概述

Roche: ?DIG Luminescent Detection Kit地高辛發(fā)光檢測試劑盒

Properties

Related Categories	12 Principles Aligned Products,?Chemical Synthesis,Greener Alternative Products,?Life Sciences
assay ?	>98% (NMR)
usage ?	sufficient for 50?blots (10 cm x 10 cm each)
mfr. no. ?	Roche
greener alternative product characteristics ?	Designing Safer Chemicals Learn more about the?Principles of Green Chemistry.
shipped in ?	dry ice
storage temp. ?	?20°C

CSPD化學(xué)發(fā)光法檢測, 靈敏度可達0.03pg同源DNA; 可檢測50張100cm2雜交膜。

?

研究領(lǐng)域:

脫氧核糖核酸

其他

詳述

Description

Application

Highly sensitive and specific detection of DIG-labeled nucleic acids in Southern and northern blots and plaque or colony lifts with anti-DIG-AP conjugate and the chemiluminescent substrate CSPD. Chemiluminescent detection with CSPD (following the instructions of the kit) is as sensitive as radioactive methods, but requires much shorter exposure times.

General description

Download the CofA, SDS and IFU specific to your product lot and format

Detection of digoxigenin-labeled nucleic acids by enzyme immunoassay with luminescence on nylon membranes.

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.? The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Packaging

1 kit containing 5 components.

Preparation Note

Working concentration:?0.5mg/ml for flow cytometry
??Storage conditions (working solution):?Antibody conjugate (vial 3): once opened, should be stored at 2 to 8°C
??Blocking Reagent (bottle 4): in solution at 2 to 8°C
??CSPD (vial 5): at 2 to 8°C when frequently used. Repeated freeze/thaw cycles should be avoided.

Enzymatic dephosphorylation of the dioxetane CSPD by alkaline phosphatase leads to the metastable phenolate anion, which decomposes and emits light at 477nm. The light emission increases with time until a constant intensity is attained.
Assay Time
??Immunological detection 1.5 hours
??Signal detection 5 to 30 minute

Note:?Store protected from light.

Specifications

Sensitivity: 0.03 pg of homologous DNA or 0.1 pg of homologous RNA is detected in a Southern or dot blot after <30-min exposure time. Single-copy genes are detected in 0.3 μg mammalian genomic DNA.

Roche地高辛標記及檢測相關(guān)試劑盒

名稱(chēng)	編號	產(chǎn)地		規格	價(jià)格￥
DIG DNA Labeling and Detection Kit 地高辛DNA標記和檢測試劑盒	11093657910?	Roche		Kit	8732
	DIG-11-dUTP隨機引物法標記DNA,?堿性磷酸酶(AP)生色反應檢測,?標記探針用于Southern、Northern和斑點(diǎn)雜交以及菌落和噬菌體篩選。組成:?未標記對照DNA1;?未標記對照DNA2; DNA稀釋液; DIG-標記對照DNA;?六核苷酸混合物; Klenow 2u/μl;?封閉液; BCIP/NBT; 10×dNTP標記混合物;?抗-地高辛-堿性磷酸酶復合物。一個(gè)Kit可用于25次標記反應和50次檢測(100cm²膜),?-20℃保存。
? DIG-High Prime DNA Labeling and Detection Starter Kit?I 高效DNA地高辛標記和檢測試劑盒I	11745832910??????	Roche		Kit	5457
	比普通DIG隨機引物法標記效率高,?操作簡(jiǎn)單,?可用于Southern、Northern和斑點(diǎn)雜交以及菌落和噬菌體篩選。一個(gè)Kit可用于12次標記反應和24次檢測反應。組成: 5×DIG-高效標記試劑;?未標記對照DNA, PBR328; DNA稀釋液;?抗-DIG-堿性磷酸酶復合物; NBT/BCIP儲存液; 10×封閉液; DIG定量復試紙條; DIG對照試紙條,?-20℃保存。
DIG-High Prime DNA Labeling and Detection Starter Kit?II 高效DNA地高辛標記和檢測試劑盒II	11585614910	Roche		Kit	5598
	比普通DIG隨機引物法標記效率高,?采用CSPD化學(xué)發(fā)光檢測靈敏度更高。一個(gè)Kit可用于12次標記反應和24次檢測反應。組成: 5×高效DIG標記復合物;?未標記對照DNA, PBR328; DNA稀釋液;?抗-DIG-堿性磷酸酶連接物; CSPD; 10×封閉液; DIG定量試紙條; DIG對照試紙條, NBT/BCIP儲存液;?-20℃保存。
? DIG DNA Labeling Kit 地高辛DNA標記試劑盒	11175033910	Roche		40T	7753
	隨機引物擴增法以DIG-11-dUTP標記DNA; DIG標記探針可用于各種膜雜交。
DIG Nucleic acid Detection Kit 地高辛核酸檢測試劑盒	11175041910	Roche		Kit	5165
	NBT/BCIP顯色法檢測,?靈敏度可達0.1pg同源DNA;?可檢測40張100cm²雜交膜。-20℃保存。
DIG Oligonucleotide?3’-End Labeling Kit 寡核苷酸3’末端地高辛標記試劑盒	3353575910	Roche		25T	7432
	通過(guò)末端轉移酶以DIG-11-ddUTP標記14-100bp的寡核苷酸探針3’端用于各種膜雜交和原位雜交。每個(gè)反應可標記100pmol的寡聚核苷酸。
DIG Oligonucleotide Tailing Kit 寡核苷酸加尾地高辛標記試劑盒	3353583910	Roche		25 T	6266
	通過(guò)末端轉移酶以DIG-11-ddUTP在寡核苷酸探針3’端加一平均長(cháng)度50bp(10-100bp)的寡核苷酸,?用于DNA/RNA膜雜交和原位雜交。每個(gè)反應可標記100pmol的寡聚核苷酸。
? DIG RNA Labeling Kit?(SP6/T7) 地高辛RNA標記試劑盒(SP6/T7)	11175025910	Roche	2′10T		7592
	由SP6或T₇?RNA聚合酶通過(guò)轉錄反應過(guò)程標記RNA,?1mg模板可產(chǎn)生約10mg標記RNA用于雜交。-20℃保存。組成: pSPT18 DNA, pSPT19 DNA,?對照DNA1,?對照DNA2, DIG標記對照RNA, NTR標記混合物,?轉錄緩沖液, RNase抑制劑,?無(wú)RNase的DNase I, SP6 RNA聚合酶, T7 RNA聚合酶。
PCR DIG Probe Synthesis Kit PCR地高辛探針合成試劑盒	11636090910	Roche		25T	5598
	標記的探針可在Southern雜交后從5-10mg的人基因組DNA檢測出單拷貝基因,?是從少量DNA模板制備大量探針的方法。
DIG Northern Starter?Kit	12039672910	Roche		10T	5052
	包含標記DIG RNA及進(jìn)行Northern印跡的所有必需試劑,?配合DIG Wash and Block Buffer Set方便進(jìn)行Northern印跡;?能作10個(gè)標記反應及10次檢測(100mm²膜)。特別適合初用者使用;? 配合提供的化學(xué)發(fā)光底物CDP-Star。
DIG Luminescent Detection Kit 地高辛發(fā)光檢測試劑盒	?11363514910	Roche		Kit	5627
	CSPD化學(xué)發(fā)光法檢測,?靈敏度可達0.03pg同源DNA;?可檢測50張100cm²雜交膜。
DIG High Prime 高效地高辛DNA標記試劑	11585 606910	Roche		40T	7838
	標記DNA探針,?只需一步操作,?標記效率較通常隨機引物法高3倍。40 T

儲存與安全

儲存:

?20°C

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地高辛發(fā)光檢測試劑盒DIG Luminescent Detection Kit